Fig. 5From: Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 systemTMS5 expression in cv. YK17 and T2 generation of tms5–1 and tms5–2 plants. a Transcript abundance as estimated by qRT-PCR in the leaves and anthers of plants grown at either 22 °C or 26 °C. Values shown in the form mean ± SD (n = 3). Columns marked with a different letter indicate statistically significant differences between means (P < 0.05). b Western blot assay of TMS5 in the panicle of plants grown at 26 °CBack to article page