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Fig. 1 | BMC Plant Biology

Fig. 1

From: Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 system

Fig. 1

CRISPR/Cas9-mediated editing of TMS5. a Vector structure of Cas9/gRNA (VK005–01). b Structure of TMS5. c The TMS5 target site aligned with the tms5–1 (a 2 nucleotides deletion) and the tms5–2 (1 nucleotide insertion) mutant sequences. LB: vector left border, Hyg: Hygromycin, 35S: CaMV 35S promoter, rU6: rice U6 promoter, gRNA: guide RNA, mpCas9: Cas9 protein, Ubi: Ubiqutin promoter, RB: vector right border, PAM: protospacer adjacent motif

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BMC Plant Biology

ISSN: 1471-2229

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