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Fig. 6 | BMC Plant Biology

Fig. 6

From: Deficiency of valencene in mandarin hybrids is associated with a deletion in the promoter region of the valencene synthase gene

Fig. 6

Transient expression analysis of Cstps1 promoter in sweet orange leaves. (a) Binary vectors used for agroinfiltration. Schematic diagram of binary plasmid used in this study: AtHSP70BP is the Arabidopsis heat shock-regulated protein 70B promoter. Fort-P is Fortune promoter. Murc-P is Murcott promoter. Murc-P (+ 12) is recovered by re-inserting the 12 bp deletion into the Murcott promoter. The 12 nucleotides are highlighted by red. GUSin, the intron-containing β-glucuronidase; NosT, the nopaline synthase gene terminator; HptII, the coding sequence of hygromycin phosphotransferase II; LB and RB, the left and right borders of the T-DNA region; (b) Via Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltration in Valencia orange leaves with blue color showing GUS expression for Fortune Cstps1 promoter (FortP-GUSin, 975 bp), Murcott Cstps1 promoter (MurcP-GUSin, 957 bp), Murcott Cstps1 promoter + the missing 12-bp (MurcP(+ 12)-GUSin, and AtHSP70BP-GUSin (Arabidopsis heat shock-regulated protein 70B promoter). The experiment was repeated twice with similar results (data not shown)

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