Fig. 6From: Deficiency of valencene in mandarin hybrids is associated with a deletion in the promoter region of the valencene synthase geneTransient expression analysis of Cstps1 promoter in sweet orange leaves. (a) Binary vectors used for agroinfiltration. Schematic diagram of binary plasmid used in this study: AtHSP70BP is the Arabidopsis heat shock-regulated protein 70B promoter. Fort-P is Fortune promoter. Murc-P is Murcott promoter. Murc-P (+ 12) is recovered by re-inserting the 12 bp deletion into the Murcott promoter. The 12 nucleotides are highlighted by red. GUSin, the intron-containing β-glucuronidase; NosT, the nopaline synthase gene terminator; HptII, the coding sequence of hygromycin phosphotransferase II; LB and RB, the left and right borders of the T-DNA region; (b) Via Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltration in Valencia orange leaves with blue color showing GUS expression for Fortune Cstps1 promoter (FortP-GUSin, 975 bp), Murcott Cstps1 promoter (MurcP-GUSin, 957 bp), Murcott Cstps1 promoter + the missing 12-bp (MurcP(+ 12)-GUSin, and AtHSP70BP-GUSin (Arabidopsis heat shock-regulated protein 70B promoter). The experiment was repeated twice with similar results (data not shown)Back to article page