Fig. 6

Novel miRNAs identified in the root of wild tomato. a The precursors of novel miRNAs identified in this study. b-d The stem-loop secondary structures of the novel miRNAs predicted by mfold (http://unafold.rna.albany.edu/?q=mfold) (c) and (d) are the zoomed regions of novel miRNAs and their corresponding miRNA*s. The nucleotides marked by the red line refer to the miRNA, whereas the nucleotides marked by the green line refer to the miRNA*. f The expression abundance of novel miRNAs identified in this study. g The relative expression of novel miRNAs in hypoxia-treated and control wild tomato root by stem-loop qRT-PCR. The two novel miRNAs were detected at the same time as they share the same mature miRNA sequence. Asterisks indicate statistically significant differences compared with control by Student’s t test (*P < 0.05; **P < 0.01)