Fig. 7

PtoMYB221 and PtoMYB156 are transcriptional repressors. a. Schematic representation of the constructs used for evaluation of the repression activity of PtoMYB221 and PtoMYB156 in dual LUC assay in poplar mesophyll protoplasts. The EAR-like motif repression domain of SUPERMAN (SUPRD) was used as a reference repressor [93]. b. Relative LUC activities after co-transformation with the reporter and effectors, where pUC18 was used as a control vector. Three independent experiments show similar results. Error bar represents standard deviation of three independent batches of P. tomentosa mesophyll protoplast transfections from one of the experiments. Significance was tested with one-way ANOVA to evaluate the roles of the effectors on the LUC expression. The different alphabets above the bar indicates statistically significant differences with p value < 0.05, while the same alphabet mean no significant differences