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Fig. 6 | BMC Plant Biology

Fig. 6

From: Endoplasmic reticulum–localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa

Fig. 6

PtoUBC34 interacts with both PtoMYB221 and PtoMYB156 in vivo. a. BiFC in poplar protoplasts demonstrated that PtoUBC34 interacts with both the PtoMYB221 and PtoMYB156 in the ER. The BiFC signals co-localized with the marker Bip:RFP in the ER, suggesting that PtoUBC34 and each of PtoMYB221 and PtoMYB156 were co-localized in the same BiFC complex in the ER. Bar = 10 μm. b. Coimmunoprecipitation analysis showed an interaction of PtoUBC34s with both the PtoMYB221 and PtoMYB156. PtoUBC34s:Flag was co-expressed with each of MYB221:Myc and MYB156:Myc in poplar protoplasts. Protein extracts were incubated with anti-Flag coupled agarose. Immunoprecipitates (IP) and input proteins were analyzed by immunoblotting using anti-Flag and anti-Myc antibodies as indicated

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