Fig. 5From: The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expressionEffects of mutations in the PB1 domain on nitrate-induced expression. Expression levels of NLP7 (a) and four nitrate-inducible genes, NIA1 (b), NIA2 (c) NRT2.1 (d) and LBD39 (e) were analyzed. Seedlings of Col, nlp6 nlp7-1, and the complementation lines expressing wild-type or mutated NLP7 were grown with ammonium as the sole N source and then treated with 10 mM nitrate for 1 h. Transcript levels were normalized against UBQ10 expression; means ± SD (n = 3) are shown. Bars marked with different letters differ significantly from each other (Tukey’s HSD test, P < 0.05)Back to article page