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Fig. 3 | BMC Plant Biology

Fig. 3

From: The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression

Fig. 3

NLP7 protein levels in the complementation lines. WT and mut indicate transgenic lines expressing wild-type and mutated NLP7, respectively. After germination, seedlings were grown on 0.5× MS agar plates for 4 days and then transferred to agar plates containing 10 mM KNO3 as an N source for 5 days. NLP7 was detected using an anti-MYC antibody that recognizes the MYC tag at the carboxy terminus of the introduced NLP7. Histone H3 served as a loading control. The numbers below the lanes show the relative transcript levels of NLP7 in these lines (Fig. 2b). A transgenic line over-expressing wild-type NLP7 under the control of the 35S promoter was used as a control for detection of the MYC tag

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