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Fig. 2 | BMC Plant Biology

Fig. 2

From: The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression

Fig. 2

Complementation of the nlp6 nlp7–1 double mutant by wild-type and mutant forms of NLP7. a The domain structure of the NLP7 protein. Core amino acid residues within the PB1 domain are labeled in blue font; these amino acid residues were replaced by alanine residues (red font) in the mutated protein. b Expression of introduced NLP7 (upper panel) and shoot fresh weight (lower panel) of the transgenic lines. nlp6 nlp7–1 double mutants were transformed with wild-type or mutated forms of the NLP7 coding sequence under the control of the NLP7 promoter. After germination, seedlings were grown on 0.5× MS agar plates for 4 days and then transferred to agar plates containing 10 mM KNO3 as a nitrogen source for 5 days. Col: wild-type Columbia seedlings. NLP7 transcript levels were normalized against UBIQUITIN10 (UBQ10) expression, and means ± SD (n = 3) are shown. Bars marked with different letters differ significantly from each other [Tukey’s honestly significant difference (HSD) test, P < 0.05]. Shoot fresh weight values are means ± SD (n = 9–10 plants). Asterisks indicate significant differences versus Col by one-way ANOVA with Dunnett’s post hoc test for multiple comparisons; **: P < 0.01 and ***: P < 0.001. c Correlations between NLP7 expression and shoot fresh weight. Error bars are SD

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