Fig. 2

Expression patterns of GhWRKY27 during leaf senescence in cotton. a Expression profiles of the GhWRKY27 gene in 15-, 25-, 35-, 45-, 55-, and 65-day-old leaves of the CCRI36 cotton variety during leaf senescence using RNA-seq data [30]. The expression values were normalized using the transcript per million clean tags (TPM) algorithm. b Transcript levels of GhWRKY27 in the early-ageing cotton variety CCRI74 and non-early-ageing cotton variety PB-12. Values represent the means ± standard deviations (SDs) from three replicates. The significance of the data was determined using Student’s t-test. “+” represents a comparison with 7 days in the same variety (⁺P < 0.05). “**” and “*” represent a comparison between the same days of different varieties (^**P < 0.01 and ^*P < 0.05)