Fig. 7

PbMYB12b-regulated flavonol biosynthesis in young pear leaves. a Flavonol glycoside contents in young ‘Zaosu’ leaves transiently overexpressing PbMYB12b. b The expression patterns of PbMYB12b and flavonol biosynthetic genes in young ‘Zaosu’ leaves transiently overexpressing PbMYB12b. c Flavonol glycoside contents in young ‘Red Zaosu’ leaves transiently silencing PbMYB12b using VIGS. d The expression patterns of PbMYB12b and flavonol biosynthetic genes in young ‘Red Zaosu’ leaves transiently silencing PbMYB12b using VIGS. The three-day-old leaves of tissue cultured pear plants were infiltrated and sampled at 48 h after infiltration. The control leaves were infiltrated by pCambia1301-GUS (OE-GUS) and original TRV2, respectively The DP fruit infiltrated by pCambia1301-GUS (OE-GUS) was the control. Data are the means ± SDs of three biological replicates. Asterisks indicate significant differences as assessed by Student’s t test: ^*P < 0.05, ^**P < 0.01