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Fig. 5 | BMC Plant Biology

Fig. 5

From: Isolation and functional validation of the CmLOX08 promoter associated with signalling molecule and abiotic stress responses in oriental melon, Cucumis melo var. makuwa Makino

Fig. 5

Transient transformation of tobacco plants with CmLOX08 promoter::GUS constructs. a Schematic representation of CmLOX08 promoter::GUS vector constructs. NPT II, neomycin phosphotransferase II gene; nos ter, nopaline synthase terminator; GUS, β- glucosidase gene. RB and LB, left and right T-DNA borders. The insertion position of the CmLOX08 promoter in the vector is indicated with restriction enzyme sites (HindIII and BamHI). b Schematic representation of the different 5’deletion CmLOX08 promoter constructs used to assay GUS activity in tobacco leaves. These constructs are based on the pBI121vector. The main cis-elements are represented with different patterns

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