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Fig. 5 | BMC Plant Biology

Fig. 5

From: GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max

Fig. 5

Expression patterns of GmBZL3 target genes in response to a BR inhibitor in combination with or without epibrassinolide. a Heatmap representation of expression patterns of different GmBZL3 targets in soybean Williams 82 under following conditions (Pcz: 5 μM Pcz for 10 days. Pcz-BL: 5 μM Pcz with 10 nM BL for 10 days. Pcz-BL-1 h: 5 μM Pcz for 10 days then with 1 μM BL for 1 h. Pcz-BL-8 h: 5 μM Pcz for 10 days then with 1 μM BL for 8 h). The expression data values were median-centered and normalized for each gene before transforming to the color scale (log2-transformed ratios). The color bar at the bottom shows the range of expression values from highest expression level (red) to lowest expression level (green). 0 is the median expression level (Black). b qRT-PCR analysis of six GmBZL3 target genes was performed using total RNA isolated from Wm82 seedlings under control. Pcz. Pcz-BL. Pcz-BL-1 h and Pcz-BL-8 h treatments. Relative gene expression levels (fold change, log2) are shown following normalization with actin (Glyma.18G290800) transcript values. Error bars represent the standard error of the mean. The y-axis represents the relative gene expression level in different samples. Three independent experiments were performed. A representative result is shown. The star (*) indicates statistically significant differences among the means (p < 0.05)

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