Skip to main content
Fig. 9 | BMC Plant Biology

Fig. 9

From: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

Fig. 9

Growth of E. coli expressing BjHMA4R. BL21 transformants contained a pEASY-Blunt E1 expression vector (negative control) and BjHMA4R, respectively. The cultures were adjusted to an OD600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective LB plates or on LB plates supplemented with 600 μM CdCl2 and 2.5 mM ZnCl2, Ni(NO3)2, Co(NO3)2 and Pb(NO3)2. After 1 day of incubation at 37 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each E. coli transformant were analyzed

Back to article page