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Fig. 8 | BMC Plant Biology

Fig. 8

From: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

Fig. 8

Dry weight of yeast and E. coli expressing BjHMA4R. a, b Yeast BY4741 and YK44 cells transformed with a pYES2 plasmid and a pYES2 plasmid that harbored BjHMA4R were grown in liquid YPD medium supplemented with 30 μM CdCl2. The cells were incubated at 30 °C for 10 h and 21 h. Fifteen milliliters of yeast fluid was collected by centrifugation and then dried at 95 °C for 48 h for determination of the dry weight. c, d, e E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or with a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in liquid LB medium supplemented with normal LB, 50 μM CdCl2 and 100 μM CdCl2. The cells were incubated at 37 °C for 10 h and 20 h. Fifteen milliliters of E. coli fluid was collected by centrifugation and then dried at 95 °C for 48 h to determine the dry weight. The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences (P < 0.05) between different cell groups under the same stress treatment

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