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Fig. 7 | BMC Plant Biology

Fig. 7

From: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

Fig. 7

Growth of yeast cells expressing BjHMA4R under Cd stress. BY4741, YK44, ycf1, cot1 and zrc1 transformants expressed pYES2 (negative control) and BjHMA4R, respectively. The cultures were adjusted to an OD600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective YPD plates or on YPD plates supplemented with 500 μM CdCl2 and 1.5 mM ZnCl2. After 3 days of incubation at 30 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each yeast transformant were analyzed

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BMC Plant Biology

ISSN: 1471-2229

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