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Fig. 6 | BMC Plant Biology

Fig. 6

From: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

Fig. 6

Subcellular localization of pUG36 (A1-A4) and pUG36-BjHMA4R (B1-B4) fusion proteins in yeast strain BY4741. Yeast cells expressing pUG36 and pUG36-BjHMA4R were cultured in SD-URA media supplemented with Glu; however, when the cells reached the stationary phase, they were then transferred to SD-URA-Met media supplemented with Glu for 12 h. A1 and B1, eGFP fluorescence signals; A2 and B2, vacuoles; A3 and B3, bright-field images; A4 and B4, overlay of GFP fluorescence signals, vacuoles and bright-field images. Bars = 5 μm

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