Fig. 10

Cd and Zn contents of yeast expressing BjHMA4R. Yeast BY4741 and YK44 cells transformed with a pYES2 plasmid or a pYES2 plasmid that harbored BjHMA4R were grown in normal liquid YPD medium overnight. Then, they were supplemented with 30 μM CdCl₂ (a, b), 1 μM CdCl₂ (c, e), 100 μM CdCl₂ (d, f), normal medium (g, i), or 200 μM ZnCl₂ (h, j). The cells were incubated at 30 °C for 30 min, 70 min, 5 h, 10 h or 21 h. The metal contents of the samples were analyzed with inductively coupled plasma-mass spectrometry (ICP-MS). The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences (P < 0.05) between cell groups under the same stress treatment