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Fig. 6 | BMC Plant Biology

Fig. 6

From: Identification and expression analysis of strigolactone biosynthetic and signaling genes reveal strigolactones are involved in fruit development of the woodland strawberry (Fragaria vesca)

Fig. 6

Expression analysis of SL biosynthetic and signaling genes, and measurement of strigol levels in the receptacle of woodland strawberry during fruit ripening. a-j Expression levels of SL biosynthetic genes FveD27 (a), FveCCD7 (b), FveCCD8 (c), FveMAX1A (d), FveMAX1B (e), FveLBO (f), SL receptor gene FveD14 (g) and signaling genes FveD3 (h), FveD53A (i) and FveD53B (j), (k) High performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC-ESI-MSn) measurement of strigol levels in the receptacle of woodland strawberry. Fruit samples were collected at the following six developmental stages: small green (SG, about 8 days post anthesis [DPA]), large green (LG, about 15 DPA), small white (SW, white pulp with green seeds, about 20 DPA), large white (LW, white pulp with red seeds, about 24 DPA), pink (P, slightly pink pulp with red seeds, about 26 DPA), and red (R, pulp all red, about 28 DPA) [38, 45, 46]. The transcript values were measured using qPCR with GAPDH as reference gene. Results were analyzed using the 2-ΔCT method.. Different letters at the top of each column indicate a significant difference at P < 0.05 (n = 3), by Student’s t-test

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