Fig. 5
Evaluation of the effect of H2O2, GSH, and AsA in GABA-induced tolerance to salinity-alkalinity at 3 d in muskmelon seedlings. a The effect of H2O2, GSH, and AsA in GABA regulated MDA content under salinity-alkalinity stress. b The effect of H2O2, GSH, and AsA in GABA regulated Fv/Fm under salinity-alkalinity stress. c Images of the Fv/Fm, the false color code depicted at the bottom of the image ranges from 0.5 (blue) to 0.8 (red). H2O foliar prespraying for 8 h under normal nutrient solution cultivation conditions, (Control); Normal nutrient solution containing 50 mM salinity-alkalinity and H2O foliar prespraying, (S); 50 mM GABA foliar prespraying for 8 h under salinity-alkalinity stress, (SG); 5 mM H2O2 foliar prespraying for 8 h under salinity-alkalinity stress, (H2O2 + S); 5 mM GSH foliar prespraying for 8 h under salinity-alkalinity stress, (GSH + S); 1 mM AsA foliar prespraying for 8 h under salinity-alkalinity stress, (AsA + S); 5 mM DMTU foliar prespraying for 8 h, then spraying 50 mM GABA, after 8 h, treatment of salinity-alkalinity stress, (DMTU+SG); 100 μM DPI foliar prespraying for 8 h, then spraying 50 mM GABA, after 8 h, treatment of salinity-alkalinity stress, (DPI + SG); 1 mM BSO foliar prespraying for 8 h, then spraying 50 mM GABA, after 8 h, treatment of salinity-alkalinity stress, (BSO + SG); 1 mM AF foliar prespraying for 8 h, then spraying 50 mM GABA, after 8 h, treatment of salinity-alkalinity stress, (AF + SG). Data were analyzed with SPSS 20 software (IBM) using Tukey’s multiple range test at a significance level of P < 0.05, and different letters above the bars indicate a significant difference. Data were expressed as the mean ± standard error of three independent biological replicates