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Fig. 6 | BMC Plant Biology

Fig. 6

From: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis

Fig. 6

Analysis of LR and AR formation in arf7 arf19 mutants expressing LBD16:GR or LBD18:GR. a Expression analysis of LBD16 and LBD16:GR in Col-0, arf7 arf19 and Pro35S:LBD16:GR/arf7arf19 (left panels) and LBD18 and LBD18:GR in Col-0, arf7 arf19 and Pro35S:LBD18:GR/arf7arf19 plants (right panels). Seven-d-old seedlings were used for real-time RT-PCR analysis. In the left panels, the relative fold changes were plotted after normalization to ACTIN7. The relative fold change represents the ratio of the transcript level in the mutant relative to the transcript level in Col-0 plants. In the right panels, copies of the transcripts were plotted per ng of total RNA after normalization to ACTIN7 RNA. Mean ± SE values were determined from three technical replicates. b Lateral root densities and number of ARs in the hypocotyls of Col-0, arf7 arf19, Pro35S:LBD16:GR/arf7arf19 and Pro35S:LBD18:GR/arf7arf19 plants. The LR densities were plotted by LR numbers (#) per unit primary root length (cm) measured from plants grown vertically for 2 weeks and shown in the top panel. Data are presented as means ± SE determined from 20 seedlings. Different letters indicate a significant difference determined by one-way ANOVA followed by Duncan’s post hoc test (P < 0.05). ARs were measured in seedlings that were grown on vertical plates for 3 d in darkness and then transferred to the 16-h photoperiod for 2 weeks with or without DEX and plotted in the bottom panel. Data are presented as means ± SE determined from 100 seedlings

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