Fig. 5

Expression analyses of LBD16 and LBD18 in hypocotyls of Col-0, arf7, arf19 and arf7 arf19 mutants. Expression of LBD16 (a) and LBD18 (b) transcripts in hypocotyls of Col-0, arf7, arf19 and arf7 arf19. Seedlings were etiolated in the dark until their hypocotyls were 6 mm long (T0) and then transferred to the light for 72 h (T72L). The seedlings were then harvested for RT-qPCR analysis. The relative fold change represents the ratio of the transcript level in the mutants relative to the transcript level in Col-0 at T0. Mean ± SE values were determined from three biological replicates (each biological replicate was estimated as the average of two technical RT-qPCR replicates). Different letters indicate a significant difference determined by one-way ANOVA followed by Duncan’s post hoc test (P < 0.05)