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Fig. 3 | BMC Plant Biology

Fig. 3

From: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis

Fig. 3

Complementation analysis of AR formation of lbd16 and lbd18. a Expression analysis of LBD16 in Col-0, lbd16 and ProLBD16:LBD16:Myc/lbd16 plants. b Expression analysis of LBD18 in Col-0, lbd18 and ProLBD18:LBD18:3XHA/lbd18 plants. Seven-d-old seedlings were used for real-time RT-PCR analysis (A and B). The relative fold changes were plotted after normalization to ACTIN7. The relative fold change represents the ratio in the mutant or transgenic plants relative to the transcript level in Col-0 plants. Mean ± SE values were determined from three technical replicates. c Number of ARs of ProLBD16:LBD16:myc/lbd16 and ProLBD18:LBD18:HA/lbd18 plants. Numbers above the lines indicate the line numbers of transgenic plants. The number of adventitious roots was analyzed as described in Fig. 2. Data are presented as means ± SE, determined from 100 seedlings. Different letters indicate a significant difference determined by one-way ANOVA followed by Duncan’s post hoc test (P < 0.05)

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