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Table 1 Cytokinin content of 8-d-old Brassica napus seedlings overexpressing the CKX2 gene. The concentration of cytokinin metabolites was measured in root, shoot and whole seedlings of the lines 35S:CKX2-1, 35S:CKX2-4, 35S:CKX2-13, and wild type. The cytokinin metabolite concentrations detected in the transgenic lines are shown as percent of those found in wild type. Absolute values and statistical analysis are shown in Additional file 1: Table S1. Percentage printed in bold indicates statistically significant differences (p < 0.05) as compared to wild type calculated by Student’s t-test

From: Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity

 

35S:CKX2-1

35S:CKX2-4

35S:CKX2-13

Root

Shoot

Seedling

Root

Shoot

Seedling

Root

Shoot

Seedling

iP-type

 iP

nd

188

42

nd

173

42

247

nd

52

 iPR

nd

nd

nd

nd

83

279

80

180

192

 iP9G

23

nd

45

20

nd

nd

14

nd

40

 iPR5′MP

162

89

58

100

160

128

101

179

94

tZ-type

 tZ

31

nd

58

31

nd

140

37

22

45

 tZR

50

42

32

83

35

45

79

59

31

 tZ9G

69

nd

10

28

nd

28

27

nd

7

 tZOG

na

33

40

na

28

27

na

nd

nd

 tZR5′MP

78

63

22

108

22

23

76

21

29

cZ-type

 cZ

38

63

51

30

33

57

26

35

55

 cZR

116

91

59

109

94

87

92

105

99

 cZ9G

90

93

124

76

86

159

79

82

98

 cZOG

60

nd

90

71

nd

nd

153

nd

nd

 cZR5′MP

290

57

96

191

94

173

475

107

106

  1. Abbreviations: tZ trans-zeatin, tZR tZ riboside, tZ9G tZ 9-N-glucoside, tZOG tZ O-glucoside, tZR5′MP tZR 5′-monophosphate, tZROG tZR O-glucoside, cZ cis-zeatin, cZR cZ riboside, cZ9G cZ 9-N-glucoside, cZOG cZ O-glucoside, cZR5′MP cZR 5′-mono phosphate, iP N6-(Δ2-isopentenyl)adenine, iPR iP riboside, iP9G iP 9-N-glucoside, iPR5′MP iPR 5′-monophosphate, nd concentration below the detection limit, na not applicable due to a concentration below detection limit in wild type