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Table 1 Amaranth proteins identified in differentially accumulated bands by nLC-MS/MS

From: Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species

Banda No. Protein Acc. No.b Mrc Exp. Mrd Theor. Mascot Scoree PM/SCf (%) emPAIg
1 Elongation factor 2 001926 97.28 94.87 116 8/11 0.30
Ribonuclease TUDOR 1 004841 109.14 58 18/20 0.38
Low-temperature-induced-like 018897 87.08 51 13/20 0.22
Phosphoenolpyruvate carboxylase 3 004468 108.39 46 5/6 0.08
2 Alpha-xylosidase 1 020003 87.98 93.92 233 9/11 0.30
Poly [ADP-ribose] polymerase 3 003773 82.76 166 12/23 0.29
Starch branching enzyme I 000673 100.56 160 16/20 0.41
Elongation factor 2 001926 94.87 132 10/17 0.34
Chaperone 1 008070 100.77 81 9/12 0.19
Aminopeptidase M1 006828 95.06 58 5/7 0.12
3 Methionine synthase 017360 78.85 78.493 529 18/30 0.39
Late embryogenesis abundant protein 013747 72.37 304 13/23 0.47
Methionine synthase 022179 89.82 219 9/16 0.17
Sucrose synthase 021141 69.92 124 8/17 0.28
Alpha-xylosidase 1 010666 100.26 111 7/9 0.19
Disulfide isomerase 015532 56.01 51 5/14 0.13
4 Vicilin-like 018839 72.74 61.21 191 8/18 0.25
Embryonic protein DC-8-like 000638 65.20 61 6/10 0.23
5 11S globulin 021282 57.85 78.10 186 16/32 0.31
Vicilin-like 018839 61.21 163 8/18 0.50
Catalase 007232 52.51 112 6/12 0.31
Glucose-6-phosphate isomerase 013135 57.20 80 5/12 0.28
6 Vicilin-like 018839 52.81 61.21 409 14/31 1.07
11S globulin 021282 78.10 356 18/34 0.69
Late embryogenesis abundant protein 001171 45.86 265 8/24 0.95
UTP-glucose-1-phosphate uridylyltransferase 008585 48.70 176 9/25 0.63
Enolase 1 001183 45.11 156 9/29 1.13
ATP synthase subunit mitochondrial-like 001716 59.31 145 7/15 0.50
Adenosylhomocysteinase 1 009349 53.89 119 8/16 0.56
Leucine aminopeptidase 1-like 014952 63.04 43 5/10 0.18
7 Vicilin-like 018839 47.21 61.21 96 3/5 0.37
Eukaryotic initiation factor 4A-9 003448 47.07 45 3/9 0.15
8 Phosphoglycerate kinase 006883 43.28 87.94 573 19/27 0.51
Phosphoglycerate kinase 019107 42.55 379 14/38 1.13
11S globulin 021282 78.10 140 14/25 0.15
Vicilin-like 018839 61.21 133 6/13 0.19
Actin-7 019031 41.93 104 7/19 0.40
9 Glyceraldehyde-3-phosphate dehydrogenase 011043 38.41 31.58 410 9/37 0.44
Vicilin-like 018839 61.21 403 10/22 0.46
Aldose 1-epimerase-like 015176 32.27 132 6/24 0.27
Dehydrin Rab18-like 003168 26.47 58 5/27 0.33
11S globulin 021282 78.10 37 5/10 0.10
10 Vicilin-like 018839 37.42 61.21 437 13/25 0.37
Lactoylglutathione lyase 011906 30.92 143 4/14 0.28
Malate dehydrogenase 021284 36.13 124 6/29 0.19
11 Vicilin-like 006304 31.21 62.07 534 15/34 0.47
Late embryogenesis abundant protein (SMP) 006906 28.70 190 6/33 0.60
Late embryogenesis abundant protein (SMP) 016810 22.64 167 5/34 1.10
11S globulin 001411 55.75 130 4/10 0.13
11S globulin 021282 78.10 108 7/16 0.09
60S ribosomal protein L6–3 005418 25.60 94 5/29 0.70
Agglutinin 007409 30.40 80 4/18 0.56
12 Oil body-associated protein 1A 009953 29.19 26.80 255 11/35 1.22
Vicilin-like 006304 62.07 186 3/9 0.12
60S ribosomal protein L7–4 008528 28.35 180 5/18 0.65
Elongation factor 1-beta 1 002577 24.87 170 3/20 0.53
Oil body-associated protein 2A 004342 25.76 98 7/29 0.99
Protein synthesis inhibitor PD-S2-like 011528 30.52 94 4/19 0.42
13 Protein synthesis inhibitor PD-S2-like 011528 27.81 30.52 362 13/42 1.39
Oil body-associated protein 2A 004342 25.76 193 6/25 0.80
14 Cysteine proteinase inhibitor 6 021786 26.87 27.78 270 11/52 1.44
Vicilin-like 018839 61.21 76 5/11 0.26
11S globulin 021282 78.10 46 5/10 0.15
15 11S globulin 001411 22.98 55.75 377 8/19 0.47
16 11S globulin 021282 21.73 78.10 290 10/16 0.51
11S globulin 001411 55.75 242 5/12 0.38
17 17.6 kDa class I heat shock protein 3 013876 17.93 17.92 182 7/46 1.18
Oleosin 5 013707 20.73 72 5/28 0.96
Oleosin 5 015343 20.46 69 4/21 0.67
18 Cyclophilin 002428 17.43 17.21 85 2/13 0.26
17.4 kDa class I heat shock protein 3 012223 17.36 34 6/33 0.25
19 Late embryogenesis abundant protein (LEA_5) 008005 15.82 9.66 152 5/59 2.50
20 Vicilin-like 018839 14.82 61.21 84 4/8 0.30
23 Histone H4 005348 11.74 11.40 62 5/41 1.51
24 Late embryogenesis abundant protein (LEA_5) 019862 10.73 8.53 49 3/49 0.47
27 Granule-bound starch synthase chloroplastic amyloplastic 011500 63.25 63.00 1112 27/58 3.08
11S globulin 021282 78.10 287 14/26 0.42
Vicilin-like 018839 61.21 231 10/23 0.48
Indole-3-aceticacid-amido synthetase 011444 70.17 65 4/6 0.21
28 Granule-bound starch synthase chloroplastic amyloplastic 011500 56.53 63.00 837 19/43 2.36
11S globulin 021282 78.10 475 17/38 1.04
Vicilin-like 018839 61.21 38 3/6 0.12
29 11S globulin 021282 52.41 78.10 412 20/43 1.22
Granule-bound starch synthase chloroplastic amyloplastic 011500 63.00 122 8/13 0.47
Vicilin-like 018839 61.21 119 10/23 0.66
ATP synthase subunit mitochondrial-like 001716 59.31 116 9/21 0.59
Late embryogenesis abundant protein 001171 45.86 114 5/13 0.35
Elongation factor 1-alpha 1 001308 50.96 66 7/17 0.50
Serine hydroxymethyltransferase 4 009350 59.74 66 4/12 0.12
30 Vicilin-like 018839 37.95 61.21 333 9/23 0.50
11S globulin 021282 78.10 210 13/23 0.50
Aldose 1-epimerase-like 015176 32.27 140 5/21 0.38
11-beta-hydroxysteroid dehydrogenase 1B 004692 74.56 109 9/18 0.21
Glyceraldehyde-3-phosphate dehydrogenase 013553 31.71 83 6/28 0.73
31 Vicilin-like 006304 19.47 62.10 90 7/17 0.20
Oil body-associated protein 1A 009953 26.79 89 7/35 1.60
32 Vicilin-like 006304 27.11 62.10 90 3/5 0.06
Protein synthesis inhibitor PD-S2-like 011528 30.52 82 8/30 0.81
Oil body-associated protein 2A 004342 25.76 45 4/20 0.32
33 Vicilin-like 018839 14.75 61.21 137 4/9 0.27
Vicilin-like 006202 59.16 109 5/7 0.28
Nucleoside diphosphate-kinase1 014404 16.19 45 3/17 0.22
11S globulin 001411 55.75 42 3/6 0.55
34 11S globulin 001411 10.72 55.75 109 6/15 0.22
Vicilin-like 006202 59.16 54 5/7 0.28
  1. aBand numbers according Fig. 5. bAccession number according to the database reported by Clouse et al. [9]. cExperimental molecular weight (kDa). dTheoretical molecular weight (kDa). eMASCOT Score, individual ion scores > 33 are statistically significant (P < 0.01), only identifications with peptide matches above identity threshold when FDR ≤5% were considered true. fPeptides Matched/Sequence Coverage. gExponentially Modified Protein Abundance Index. Protein names in bold letters are discussed in the text