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Table 1 The yield, monosaccharide compositions (mol%) and degree of methyl esterification of cell walls and HEPES fractions from celery collenchyma strands at different stages of development

From: Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles

Samples

Stage of development

Petiole length (cm)

Yield (%)

Monosaccharides

TS (μg/mg)

DM

Rha

Fuc

Ara

Xyl

Man

Gal

Glc

C-Glc

UA

CWs

1

2–5

7.1

2.2d

0.8a

9.1d

6.2a

2.0a

9.4b

2.0a

28.0a

40.3b

950a

36.1b

2

10–15

7.8

1.9c

0.9b

6.5c

6.7b

2.6b

10.3b

2.1b

31.9b

37.0a

982ab

35.7b

3

20–25

10.2

1.7b

0.9b

4.3b

7.7c

2.9c

9.5b

2.2c

33.7c

36.7a

951a

34.9b

4

35–40

12.8

1.5a

0.9b

2.5a

7.9c

3.0c

4.7a

2.2c

35.8d

41.5b

1013b

19.2a

HEPES fraction

1

2–5

2.4

2.4a

4.6c

15.5d

9.9a

4.5c

22.8c

9.8c

ND

30.5a

183a

ND

2

10–15

1.7

2.6a

1.1b

11.4c

12.9b

2.8a

16.5b

5.6b

ND

47.1c

436b

ND

3

20–25

1.1

2.5a

0.9ab

10.1b

16.6c

3.0b

16.0ab

6.6a

ND

44.3b

420b

ND

4

35–40

0.9

2.8a

0.6a

9.2a

17.3d

2.9b

14.9a

6.3a

ND

46.0bc

435b

ND

  1. The yield of the CWs and HEPES fraction is the freeze-dried weight recovered from 100 g fresh weight of collenchyma strands
  2. Rha and Glc values are from the 2 M TFA hydrolysis; all other neutral sugars values are from H2SO4 hydrolysis
  3. Values are the average of duplicates or triplicates (n = 2 or 3)
  4. Different letters (a, b, c) indicate significant differences (P ≤ 0.05)
  5. ND not determined, DM degree of methyl esterification of pectin (mol%), Rha Rhamnose, Fuc fucose, Ara arabinose, Xyl xylose, man mannose, Gal galactose, Glc non-cellulosic glucose from TFA hydrolysis, C-Glc cellulose glucose, TFA glucose subtracted from H2SO4 glucose, UA uronic acids, TS total monosaccharides, sum of uronic acid and neutral monosaccharides