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Fig. 4 | BMC Plant Biology

Fig. 4

From: Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles

Fig. 4

Immunofluorescence micrographs of transverse sections from celery collenchyma strands at four stages of development indirectly labelled with the monoclonal antibodies LM15, LM10, LM11 and LM21. LM15 (a-d), sections were labelled without pretreatment with pectate lyase before labelling with LM15; LM15+ PL (e-h), LM10+ PL (i-l), LM11+ PL (m-p), LM21+ PL (q-t), sections were pretreated with pectate lyase before labelling with LM15, LM10, LM11 and LM21, respectively. Stage 1 (2.6 cm long petiole) (a, e, i, m, q); Stage 2 (11 cm petiole) (b, f, j, n, r); Stage 3 (24 cm petiole) (c, g, k, o, s); Stage 4 (40 cm petiole) (d, h, l, p, t). The insets show the collenchyma cells marked with arrows in the main panel at higher magnifications. The intense labelling in the insets was marked with arrows, double headed arrows, arrowheads and double arrows. Arrows indicate the middle lamella junctions or cell corners, double headed arrows indicate the middle lamella, and arrowheads indicate the inner region of the walls, e = epidermis, c = collenchyma cells, p = parenchyma cells. Scale = 50 μm in the main panel. Scale = 25 μm in the insets

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