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Fig. 3 | BMC Plant Biology

Fig. 3

From: Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles

Fig. 3

Immunofluorescence micrographs of transverse sections of collenchyma strands at four stages of development indirectly labelled with the monoclonal antibodies LM19 (a-h), LM20 (i-l), LM5 (m-p) and LM6 (q-t). LM19 + Na2CO3, sections were pretreated with 0.1 M Na2CO3 before labelling with LM19. Stage 1 (2.6 cm long petiole), (a, e, i, m, q); Stage 2 (11 cm long petiole), (b, f, j, n, r); Stage 3 (24 cm long petiole), (c, g, k, o, s); Stage 4 (40 cm long petiole), (d, h, I, p, t). The insets show the collenchyma cells marked with arrows in the main panel at higher magnifications. The intense labelling in the insets were marked with arrows, double headed arrows, arrowheads and double arrows. Arrows indicate the middle lamella junctions or cell corners, double headed arrows indicate the middle lamella, arrowheads indicate the inner region of the walls, and stars indicate the artefacts (strong labelling originates from collapse of walls). A dashed-line circle indicates the absence of labelling at the centre of a cell corner. e = epidermis, c = collenchyma cells, p = parenchyma cells. Scale = 50 μm in the main panel and = 25 μm in the insets

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