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Fig. 3 | BMC Plant Biology

Fig. 3

From: Involvement of G6PD5 in ABA response during seed germination and root growth in Arabidopsis

Fig. 3

G6PD5 affects the ROS levels with ABA treatment and the activities and transcript levels of antioxidant enzymes in Arabidopsis seedlings. 5-day-old seedlings were grown vertically on 1/2 MS agar plates supplemented with the 10 μM ABA for 6 h. a Staining of roots with DAB (brown color displaying H2O2) after ABA treatment. Bar = 100 μm. b Staining of roots with NBT (blue color displaying O2.-) after ABA treatment. Bar = 50 μm. c and d The activities of antioxidant enzymes and transcript levels of antioxidant enzyme responsive genes. The transcript levels were normalized to Actin2. e The seeds and seedlings are incubated with 0.25 μM ASC or 5 μM GSH

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