Fig. 3From: Involvement of G6PD5 in ABA response during seed germination and root growth in ArabidopsisG6PD5 affects the ROS levels with ABA treatment and the activities and transcript levels of antioxidant enzymes in Arabidopsis seedlings. 5-day-old seedlings were grown vertically on 1/2 MS agar plates supplemented with the 10 μM ABA for 6 h. a Staining of roots with DAB (brown color displaying H2O2) after ABA treatment. Bar = 100 μm. b Staining of roots with NBT (blue color displaying O2.-) after ABA treatment. Bar = 50 μm. c and d The activities of antioxidant enzymes and transcript levels of antioxidant enzyme responsive genes. The transcript levels were normalized to Actin2. e The seeds and seedlings are incubated with 0.25 μM ASC or 5 μM GSHBack to article page