Fig. 4

2DE images of embryo and endosperm during seed germination in Zhengmai 366. Numbered spots indicate differentially accumulated protein (DAP) spots identified by MALDI-TOF/TOF-MS with significant accumulation changes under salt stress. a Embryo gel, samples were extracted from the embryo and electro focused on an 18 cm pH 3–10 linear IPG strip. b Endosperm gel, samples were extracted from the endosperm and electro focused on an 18 cm pH 3–10 linear IPG strip. The different colours of protein spots are corresponding to protein expression patterns shown in Fig. 7. The red spots: Cluster I; the orange spots: Cluster II; the yellow spots: Cluster III; the green spots: Cluster IV; the black spots: Cluster V; the blue spots: Cluster VI; the purple spots: Cluster VII; the light blue spots: Cluster VIII