Table 6 BestKeeper analysis of reference gene expression stability in infected leaves
Factors | Gene | |||||
|---|---|---|---|---|---|---|
EF1 | EF2 | EF3 | UBC | UBCE2 | UBQ | |
Geo mean [Ct] | 24.98 | 22.32 | 24.43 | 24.80 | 25.10 | 25.83 |
Ar mean [Ct] | 24.99 | 22.32 | 24.44 | 24.80 | 25.18 | 25.84 |
min [Ct] | 24.41 | 21.99 | 23.94 | 24.59 | 24.79 | 25.18 |
max [Ct] | 25.69 | 22.48 | 25.30 | 25.03 | 25.57 | 26.97 |
SD [Ct] | 0.35 | 0.17 | 0.43 | 0.16 | 0.20 | 0.63 |
CV[% Ct] | 1.40 | 0.74 | 1.76 | 0.63 | 0.81 | 2.42 |
Coeff. of corr.[r] | 0.72 | 0.79 | 0.98 | 0.08 | −0.56 | 0.89 |
Coeff. of det.[r2] | 0.52 | 0.62 | 0.96 | 0.01 | 0.32 | 0.79 |