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Table 5 BestKeeper analysis of reference gene expression stability in urediniospores and germinated spores

From: Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia helianthi Schw.

Factors (a)

Gene

EF1

EF2

EF3

UBC

UBCE2

UBQ

Geo mean [Ct]

23.60

18.87

21.38

21.92

25.68

25.12

Ar mean [Ct]

23.61

18.88

21.39

21.93

25.72

25.14

min [Ct]

22.76

18.17

20.92

21.22

24.17

23.72

max [Ct]

24.99

19.44

22.15

22.55

27.31

26.37

SD [Ct]

0.77

0.54

0.45

0.61

1.52

0.83

CV[% Ct]

3.26

2.84

2.11

2.80

5.92

3.31

Coeff. of corr.[r]

0.92

0.99

0.93

1.00

0.99

0.88

Coeff. of det.[r2]

0.84

0.99

0.87

0.99

0.97

0.77

  1. aGeo mean [Ct]: Geometric mean of Ct value; Ar mean [Ct]: Arithmetic mean of Ct value; SD [Ct]: Standard deviation of Ct value; CV [%Ct]: Coefficient of variation of Ct value; Coeff. of corr. [r]: Coefficient of correlation r; Coeff. of det. [r2]: Coefficient of determination r2 between the reference gene and BestKeeper. The same as below