Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia helianthi Schw.

Table 2 The primer sequences and amplification efficiency of candidate reference genes

Gene symbol	Forward primer(5′~ 3′)	Reverse primer(5′~ 3′)	Tm (°C)	Amplicon size (bp)	Application efficiency (%)
ACTB	TGGTTTGGAAGCATCCGGTA	TGTCGGAGATGCCCGAATAC	56	134	94.7
EF1	CCTTCACCCCTCTGTACTGC	CCACATGTTTGGGCGGATTG	56	113	86.5
EF2	TGGTCATCCGAACGACAAGG	GCCTAGGATGCCGTAAGGAC	57	130	99.0
EF3	ATGTCTGCCGATGAAGCCAA	AAGTCGGACGAAGGCTTGAG	57	132	98.1
RPS24	TGCTTTCGCTGGAGTAGACG	CAGTTCATCCCGGTCCTACC	56	107	97.8
RPS5	GGCAAGTAGCGTGCGAATTT	GACACATGGGTGGAGACGAA	56	149	98.9
TUBA	GACACCAAGACAGACACAGGT	TCTTTGCTTTCAACCACACCC	56	102	99.5
TUBB	GCCAGAAAGAAGTTGCGACC	TTCTCCAGTTTGACTGGCCG	56	141	101.7
UBC	GTGGTGACCAAAGACTGAGACA	CGACAGTCAGCCAACCTACC	56	119	95.0
UBCE2	GTTCTAGCGGGGTTTGTGGA	TTGCAGAAAAGCCCATTCGC	57	149	101.5
UBQ	GGCAGAGGACCACAAAGTCA	GGGATGTACACCTGTGAGCC	56	105	93.1

ACTB β-actin, EF1 Elongation factor 1, EF2 Elongation factor 2, EF3 Elongation factor 3, RPS24 Ribosomal protein S24, RPS5 Ribosomal protein S5, TUBA α-tubulin, TUBB β-tubulin, UBC Ubiquitin conjugating enzyme, UBCE2 E2 ubiquitin-conjugating enzyme, UBQ Polyubiquitin

ISSN: 1471-2229