Fig. 1
CMV vector constructs and illustrations of phenotypes of the virus-infected plants and its progenies. a Diagram of CMV-A1vector constructs. The recombinant CMV-A1 vectors contained the CaMV 35S promoter segments of three different sizes (positions relative to the transcription start site − 345 to + 1, − 208 to + 1 and − 116 to + 1). A1, B2 and B4 are sequence domains that affect promoter activity of the CaMV 35S sequences. b Schematic illustration of virus-infected plants and its progenies. Nicotiana benthamiana16c plants were inoculated with CMV-A1 vectors containing a CaMV 35S promoter fragment (A1–345, A1–208 or A1–116), and then first (S1) and second (S2) self-fertilized progenies were obtained. The phenotype segregation is indicated in parenthesis. The red color represents appearance of chlorophyll autofluorescence as a consequence of the loss of GFP fluorescence by TGS, while the yellow color represents combined expression of chlorophyll autofluorescence and GFP fluorescence