Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)

Table 1 Summary statistics for 25 polymorphic microsatellite loci developed for Lychnis kiusiana. Number of alleles (N_A), observed (H_O) and expected (H_E) heterozygosities, inbreeding coefficient (F_IS), Hardy-Weinberg equilibrium (HWE), and polymorphism information content (PIC). Significant values after false discovery rate correction are indicated with asterisks (* p < 0.05, ** p < 0.01)

Locus	Size range (bp)	N _A	Fnull	H _O	H _E	F _IS	HWE	PIC
LKI04	201–217	4	0.00000	0.37255	0.36905	−0.010	0.2921	0.341
LKI05	230–260	6	0.02708	0.68627	0.72821	0.058	0.0108*	0.672
LKI08	211–229	6	0.08018	0.52941	0.64221	0.177	0.0942	0.576
LKI09	119–173	15	0.03127	0.76471	0.77927	0.019	0.0253*	0.755
LKI10	146–176	7	0.00000	0.82353	0.73986	−0.114	0.3221	0.695
LKI12	124–157	10	0.00000	0.84314	0.80431	−0.049	0.8835	0.767
LKI15	198–240	8	0.01274	0.78431	0.82702	0.052	0.7256	0.798
LKI16	222–240	5	0.02202	0.68627	0.68744	0.002	0.1274	0.625
LKI17	183–219	12	0.00013	0.72549	0.75306	0.037	0.6855	0.718
LKI20	241–268	8	0.12785	0.54902	0.77053	0.290^†	0.0006**	0.742
LKI28	158–188	8	0.00000	0.82353	0.75791	−0.074	0.0502	0.725
LKI29	211–253	10	0.00000	0.90196	0.80897	−0.116	0.0756	0.777
LKI32	226–244	4	0.00003	0.64706	0.64007	−0.011	0.5823	0.572
LKI33	126–152	8	0.05123	0.64706	0.75908	0.149	0.0593	0.711
LKI34	181–208	9	0.05066	0.7451	0.82411	0.097	0.0215*	0.797
LKI35	185–197	5	0.00001	0.56863	0.56824	−0.046	0.6829	0.414
LKI38	151–169	6	0.00000	0.7451	0.70511	−0.057	0.6523	0.653
LKI39	227–236	4	0.00002	0.56863	0.56824	−0.001	0.3841	0.510
LKI41	195–210	5	0.00679	0.70588	0.70258	−0.005	0.5343	0.649
LKI42	127–130	2	0.05875	0.39216	0.48146	0.187	0.2406	0.363
LKI43	197–203	3	0.00001	0.13725	0.13143	−0.045	1.0000	0.126
LKI44	245–248	2	0.00005	0.5098	0.50476	−0.010	1.0000	0.375
LKI45	168–192	9	0.00000	0.84314	0.76199	−0.108	0.8721	0.718
LKI46	213–237	7	0.00000	0.60784	0.60435	−0.006	0.2555	0.559
LKI48	251–279	10	0.07888	0.62745	0.70879	0.116	0.0291*	0.656
Total		174
Mean		6.96		0.6455	0.6617	0.023		0.6118

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