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Fig. 5 | BMC Plant Biology

Fig. 5

From: The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding

Fig. 5

The interaction of actin with β-1,3-glucanase associated with the accumulation of a protein-network. (A) The interaction of actin with β-1,3-glucanase was verified by pull-down. (a) The Tricine-SDS-PAGE profiles of His-tagged actin (1), the clarified supernatant of B-serum dialysed against TBS buffer (25 mM Tris-HCl + 150 mM NaCl, pH 7.2) (2), the elution solution of the His-tagged actin-binding resin (3), the prey flow-through solution of the His-tagged actin-binding resin (4) and the elution solution of the control (5). Protein was loaded at 1.3, 4.3 and 4.3 μg for lanes 1, 2 and 4, respectively;10 μL of elution solution was loaded for lanes 3 and 5. M, protein marker. (b-c) Western blotting analysis of their duplications with polyclonal antibodies raised against His-tagged actin (b) and β-1,3- glucanase (c). (B) The effect of cytochalasin B on the duration of latex flow in the field. TR, treated with dimethyl sulfoxide (DMSO) containing 0.1% cytochalasin B; CK, treated with dimethyl sulfoxide (DMSO) only as the control

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BMC Plant Biology

ISSN: 1471-2229

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