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Fig. 4 | BMC Plant Biology

Fig. 4

From: The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding

Fig. 4

Identification of the interaction of β-1,3-glucanase with chitinase, hevein and pro-hevein by SPR binding response. (A) Purity identification of the β-1,3-glucanase (Gluc), chitinase (Chit), hevein (Heve) and pro-hevein (ProH) by Tricine-SDS-PAGE. M, protein marker. Loaded were 1.8 μg of Gluc, 0.8 μg of Chit, 3.2 μg of Heve and 0.37 μg of ProH. (B) SPR-based detection of protein binding activity. The binding kinetics of β-1,3-glucanase to hevein (a), chitinase (b) and pro-hevein (c) were obtained by respectively injecting concentrations of hevein ranging from 5.25 to 168 μM, chitinase from 0.265 to 8.5 μM and pro-hevein from 5.62 to180 μM over the β-1,3-glucanase immobilized channel and reference channel at 30 μL/min for 3 min simultaneously. (C) The apparent constants Ka (association rate constant), Kd and KD calculated according to the Langmuir binding fitting model

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BMC Plant Biology

ISSN: 1471-2229

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