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Fig. 3 | BMC Plant Biology

Fig. 3

From: The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding

Fig. 3

Effect of pH value on the interactions of the primary proteins from B-serum. (A) Size-based chromatography profiles of B-serum eluted by acidic washing buffer (50 mM sodium acetate+ 25 mM NaCl, pH 5.5) (a), and the protein profiles of Tricine-SDS-PAGE (b). (B) Size-based chromatography profiles of B-serum eluted by neutral washing buffer (50 mM Tris-HCl + 25 mM NaCl, pH 7.2) (a), and the protein profiles of Tricine-SDS-PAGE (b). B-S, B-serum. 10 μg of protein was loaded for the B-serum lane. The number above the lane is the fraction number. 10 μL of collection was loaded for each lane. M, protein marker. (C) The protein profiles of Tricine-SDS-PAGE (a) for the condensed fractions of peak 1 (1) and peak 3 (2) that were obtained by elution with the neutral buffer and western blotting (b-d) with the polyclonal antibodies raised against β-1,3-glucanase (b), chitinase (c) and hevein (d). For lanes 1 and 2, 4 and 3 μg of protein was loaded, respectively. M, protein marker; Gluc, β-1,3-glucanase; Chit, chitinase; Heve, hevein; ProH, pro-hevein

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BMC Plant Biology

ISSN: 1471-2229

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