Fig. 2From: Mapping of a novel clubroot resistance QTL using ddRAD-seq in Chinese cabbage (Brassica rapa L.)The clubroot disease resistance linkage map identified in chromosome A08 from Chinese cabbage (Brasssica rapa var. pekenensis) (a). The mapped locus is resistant to the pathotype 4 isolate Seosan. The SNP haplotyping was done by ddRAD-seq using a mapping population containing 45 resistant and 45 susceptible F2 genotypes and 3 resistant and 3 susceptible parents. ddRAD-seq was accomplished using 2152 markers. QTL mapping was done using Windows QTL cartographer with 1000 permutations and a significance level of 0.05. Four HRM probes separated resistant and susceptible members of the F2 population (b), see Additional file 6: Figure S1Back to article page