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Fig. 4 | BMC Plant Biology

Fig. 4

From: Analysis of ambient temperature-responsive transcriptome in shoot apical meristem of heat-tolerant and heat-sensitive broccoli inbred lines during floral head formation

Fig. 4

Growth and the expression of flowering-associated genes in the heat-tolerant and heat-sensitive broccoli genotypes. a Growth of the two broccoli lines at 22 °C at different times post germination (18, 28, 38, 48, 58 and 68 days). d, day. b RT-PCR analysis of flowering-associated genes at different growth stages in two broccoli genotypes. The cDNA sequences of BoFLC1–5, BoFT, BoSOC1 and Bo18S with their corresponding accession numbers were obtained from GenBank of National Center for Biotechnology Information (Additional file 9: Table S7). The primers were designed to amplify these gene transcripts specifically. Bo18s was used as an internal control for RT-PCR analysis. White bar equals 4 cm

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