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Fig. 7 | BMC Plant Biology

Fig. 7

From: CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance

Fig. 7

CaHDZ27 can bind the motif CAATTATTG in the promoter ofCaLRR-RLK1 andactivateCaLRR-RLK1 transcription. a Scheme of the CaLRR-RLK1 promoter region, primers used in ChIP-qPCR assay were marked by arrows. b ChIP-qPCR assays were performed using the specific primers corresponding to the region containing the motif (CAATTATTG). c The transcript levels of CaLRR-RLK1 in HA-CaHDZ27-SRDX or HA-CaHDZ27 transiently overexpressed pepper leaves. d The transcript levels of CaLRR-RLK1 in CaHDZ27-silenced leaves challenged by R. solanacearum. e GUS activities driven by the promoter of CaLRR-RLK1 were assayed when transiently overexpressing HA-CaHDZ27-SRDX or HA-CaHDZ27 in pepper leaves. f Activities of GUS driven by the pCaLRR-RLK1 or pCaLRR-RLK1mut,in which CAATAATTG was replaced by CAGGGGTTG, when co-transiently overexpressed withHA-CaHDZ27 in pepper leaves. In b, c and e, the values were the mean ± standard error (n = 3), different letters indicate significant differences, as determined by LSD test (P < 0.05). In d and f, asterisks indicate significant difference according to Student’s t test at P < 0.05

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BMC Plant Biology

ISSN: 1471-2229

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