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Fig. 4 | BMC Plant Biology

Fig. 4

From: CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance

Fig. 4

Silencing of CaLRR-RLK1enhanced susceptibility of pepper plants to RSI. a The efficiency of CaLRR-RLK1 by VIGS was detected by qRT-PCR in TRV:CaLRR-RLK1 and TRV:00 plants challenged by R.solanacearum. b Disease symptoms of pepper plants at 10 dpi with R. solanacearum. c Disease index of R. solanacearum were evaluated daily for 14 days using a scale of 0 to 4. At least 50 plants were inoculated with R. solanacearum by root irrigation for TRV:CaLRR-RLK1 or TRV:00 plants. Results shown are the means of three independent experiments, each with 10 plants for TRV:CaLRR-RLK1 orTRV:00,bars indicate standard error of the mean. d Growth of R. solanacearum in stems of TRV:CaLRR-RLK1 and TRV:00 plants at 9 days post inoculation of R. solanacearum by root irrigation. e Growth of R. solanacearum in leaves of TRV:CaLRR-RLK1 and TRV:00 plants at 3 days post leaf-inoculation of R. solanacearum. f The relative transcript levels of the defense related genes in leaves of TRV:CaLRR-RLK1andTRV:00 plants. In a, d, e and f, values were the mean ± standard error (n = 6). Asterisks (P < 0.05) indicate significant differences as analyzed by the Student’s t-test

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BMC Plant Biology

ISSN: 1471-2229

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