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Table 2 IC50 for ADP, NAG and G6P for maize HXK activity

From: Biochemical properties and subcellular localization of six members of the HXK family in maize and its metabolic contribution to embryo germination

 

IC50 (mM)

 

ADP

NAG

G6P

ZmHXK4Δ30

0.71 ± 0.15

52.14 ± 16.42

47.49 ± 2.89

ZmHXK5Δ30

0.69 ± 0.15

46.80 ± 1.82

47.46 ± 1.44

ZmHXK6Δ30

0.72 ± 0.14

25.15 ± 2.25

53.54 ± 2.48

ZmHXK7

6.47 ± 1.14

15.01 ± 3.24

IS

ZmHXK8

3.12 ± 1.14

37.54 ± 1.63

IS

  1. IS Insensitive, since the HXK activity was similar to the control
  2. HXK activity was determined using 5 mM Glc and 2 mM ATP, except for ZmHXK8 in which 50 mM Glc was used. The half maximal inhibitory values (IC50) were obtained with Origin 8.0 software and the values represent the average ± standard deviation of two technical repeats from three independent replicates
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BMC Plant Biology

ISSN: 1471-2229

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