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Table 2 IC50 for ADP, NAG and G6P for maize HXK activity

From: Biochemical properties and subcellular localization of six members of the HXK family in maize and its metabolic contribution to embryo germination

  IC50 (mM)
  ADP NAG G6P
ZmHXK4Δ30 0.71 ± 0.15 52.14 ± 16.42 47.49 ± 2.89
ZmHXK5Δ30 0.69 ± 0.15 46.80 ± 1.82 47.46 ± 1.44
ZmHXK6Δ30 0.72 ± 0.14 25.15 ± 2.25 53.54 ± 2.48
ZmHXK7 6.47 ± 1.14 15.01 ± 3.24 IS
ZmHXK8 3.12 ± 1.14 37.54 ± 1.63 IS
  1. IS Insensitive, since the HXK activity was similar to the control
  2. HXK activity was determined using 5 mM Glc and 2 mM ATP, except for ZmHXK8 in which 50 mM Glc was used. The half maximal inhibitory values (IC50) were obtained with Origin 8.0 software and the values represent the average ± standard deviation of two technical repeats from three independent replicates