Protein | Parametera | Substrate | |||
---|---|---|---|---|---|
Glucose | Fructose | Mannose | ATP | ||
ZmHXK4Δ30 | V max | 5.65 ± 0.12 | 3.05 ± 0.46 | 3.23 ± 0.22 | 1.83 ± 0.35 |
K m | 195.70 ± 31.96 | 19,324.15 ± 3914.89 | 138.85 ± 22.42 | 134.90 ± 25.74 | |
k cat /K m | 2.85 × 104 | 1.55 × 102 | 2.29 × 104 | 1.34 × 104 | |
ZmHXK5Δ30 | V max | 4.55 ± 0.34 | 2.49 ± 0.29 | 2.77 ± 0.19 | 1.54 ± 0.56 |
K m | 136.65 ± 20.01 | 30,216.8. ± 5708.76 | 119.55 ± 33.30 | 135.60 ± 11.46 | |
kcat/Km | 3.27 × 104 | 81.00 | 2.28 × 104 | 1.12 × 104 | |
ZmHXK6Δ30 | V max | 3.60 ± 0.14 | 2.61 ± 0.47 | 3.08 ± 0.33 | 1.08 ± 0.32 |
K m | 65.86 ± 15.19 | 4291.84 ± 1410.04 | 48.85 ± 1.06 | 198.85 ± 12.80 | |
k cat /K m | 5.40 × 104 | 6.01 × 102 | 6.23 × 104 | 5.36 × 103 | |
ZmHXK7 | V max | 27.19 ± 0.77 | 23.44 ± 0.30 | 21.33 ± 0.54 | 13.21 ± 0.48 |
K m | 108.10 ± 17.96 | 2701.85 ± 590.93 | 123.79 ± 11.61 | 28.74 ± 0.30 | |
k cat /K m | 2.46 × 105 | 8.48 × 103 | 1.68 × 105 | 4.49 × 105 | |
ZmHXK8 | V max | 17.38 ± 0.24 | NC | 14.79 ± 0.99 | 10.91 ± 0.34 |
K m | 4687 ± 299.53 | NC | 10,396.50 ± 54.31 | 147.20 ± 62.08 | |
k cat /K m | 3.66 × 103 | NC | 1.40 × 103 | 7.32 × 104 |