Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: Biochemical properties and subcellular localization of six members of the HXK family in maize and its metabolic contribution to embryo germination

Fig. 4

Saturation curves of HXK activity of ZmHXK7 and ZmHXK8 for glucose, fructose, mannose and ATP. The HXK activity of recombinant proteins (a, b, c, d) ZmHXK7 and (e, f, g, h) ZmHXK8 were assayed in a reaction medium with a fixed concentration of ATP (2 mM) and increasing concentration of Glc (A, E), Fru (B, F) and Man (C y G). To determine the Km for ATP (D, H) 5 mM Glc for ZmHXK7 and 50 mM Glc for ZmHXK8 were included in the reaction medium. The data was adjusted to Michaelis-Menten equation, except for ZmHXK8 which did not reach the saturation with fructose as substrate even in high concentrations. The graphics were obtained with the Origin 8.0 software. Values represent the average ± standard deviation of two technical repeats from three independent replicates

Back to article page