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Fig. 3 | BMC Plant Biology

Fig. 3

From: Biochemical properties and subcellular localization of six members of the HXK family in maize and its metabolic contribution to embryo germination

Fig. 3

Saturation curves of HXK activity of ZmHXK4Δ30, ZmHXK5Δ30 and ZmHXK6Δ30 for glucose, fructose, mannose and ATP. HXK activity of maize isoenzymes was determined for the recombinant truncated versions: a, b, c, d ZmHXK4Δ30, (e, f, g, h) ZmHXK5Δ30, (i, j, k, l) ZmHXK6Δ30. The reaction medium contained 2.5 μg of purified recombinant protein. The effect of increasing concentrations of Glc (a, e, i), Fru (b, f, j) and Man (c, g, k) was determined with a fixed concentration of 2 mM ATP. To determine the Km for ATP (d, h, l) 5 mM Glc was included in the reaction medium. The data was adjusted to Michaelis-Menten equation. The graphics were obtained with the Origin 8.0 software. Values represent the average ± standard deviation of two technical repeats from three independent replicates

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