Fig. 2

Purified recombinant full and truncated protein versions of maize HXKs, ZmHXK4–9, and the comparison of the N-terminal sequences of the HXKs from A. thaliana, rice and maize. a Western blot of purified recombinant full HXKs (b) N-terminal sequences from HXKs from A. thaliana, rice and maize. The sequence analysis was made by inspection with the aid of the software SeaView [33]. The hydrophobic sequence is approximately 20 amino acids long in several ZmHXKs and it is highly conserved among other HXKs. c Western blots of full and truncated HXK versions that were used to determine the kinetic parameters. The recombinant proteins have a V5 epitope which was detected using the anti-V5-HRP (R961–25, Invitrogen)