Transcriptomic signature reveals mechanism of flower bud distortion in witches’-broom disease of soybean ( Glycine max )

Sarika Jaiswal; Pravin V. Jadhav; Rahul Singh Jasrotia; Prashant B. Kale; Snehal K. Kad; Mangesh P. Moharil; Mahendra S. Dudhare; Jashminkumar Kheni; Amit G. Deshmukh; Shyamsundar S. Mane; Ravindra S. Nandanwar; Suprasanna Penna; Joy G. Manjaya; Mir Asif Iquebal; Rukam Singh Tomar; Prashant G. Kawar; Anil Rai; Dinesh Kumar

doi:10.1186/s12870-018-1601-1

Table 5 Gene and primes sequence details of qPCR

From: Transcriptomic signature reveals mechanism of flower bud distortion in witches’-broom disease of soybean (Glycine max)

Gene Name	Gene ID	Forward primer	Tm	Reverse primer	Tm
Receptor like kinase isoform X1	RLKI-X1	ACCAAACTCGGAACTTGATGGAGC	63.37	GTAGGCCTAAGTGTTGGAGAAGCA	62.37
Transcript ID: TRINITY_DN54263_c0_g1_i14	RLKI-X1	ACCAAACTCGGAACTTGATGGAGC	63.37	GTAGGCCTAAGTGTTGGAGAAGCA	62.37
La-related protein	LRP	GGCCCATTCCATTTCCAGCACG	64.61	TGCACATGAGGAAGAAGATGGGGA	63.71
Transcript ID: TRINITY_DN56761_c0_g1_i4	LRP	GGCCCATTCCATTTCCAGCACG	64.61	TGCACATGAGGAAGAAGATGGGGA	63.71
Probable peroxygenase 4 (Glycine max)73,458,878 (putative peroxygenase 4	PPER4	GCTTCCATCATAAACACTTCGG	62.13	AGGAAGGATTGGTGGCTTGGTT	64.61
Transcript ID: TRINITY_DN61766_c0_g1_i1	PPER4	GCTTCCATCATAAACACTTCGG	62.13	AGGAAGGATTGGTGGCTTGGTT	64.61
Zinc finger CCH domain containing protein 69 –like isoform X1	ZF	GAGCCTGTCTGAAAGGGGAGCA	64.24	TGCAGCGACTACCATAAGCACA	62.3
Transcript ID: TRINITY_DN56363_c0_g1_i13	ZF	GAGCCTGTCTGAAAGGGGAGCA	64.24	TGCAGCGACTACCATAAGCACA	62.3
AP2 like ethylene responsive transcription factor ANT like	AP2TF	ACTGTGGGGTGTGGAGAGTTGCA	66.09	GCCCTCTCTTCTTTGCATCCACAGC	66.04
Transcript ID:
TRINITY_DN55029_c2_g4_i10
Glyceraldehyde 3 phosphate dehyadrogenase 2,cytolistic	G3PDC	GCCCTCTGACTCCTCCTTGATAGCA	65.42	GGCATTCCGTGTCCCTACTGTGGA	66.28
Transcript ID: TRINITY_DN8658_c0_g1_i1	G3PDC	GCCCTCTGACTCCTCCTTGATAGCA	65.42	GGCATTCCGTGTCCCTACTGTGGA	66.28
Cellulose synthase A catalytic subunit	CS	AACTCACCAGACATCGGTTGCCC	65.11	AAGTCGGGGATGCTGTGGGAAGA	65.72
Transcript ID: TRINITY_DN51118_c0_g1_i8	CS	AACTCACCAGACATCGGTTGCCC	65.11	AAGTCGGGGATGCTGTGGGAAGA	65.72
Auxin response Factor 3	ARF3	CCCATTTCATGTGACTCTTCTG	63.63	TGGATTCCAAAGAGCTGAACCT	61.5
Transcript ID: TRINITY_DN22465_c0_g1_i1	ARF3	CCCATTTCATGTGACTCTTCTG	63.63	TGGATTCCAAAGAGCTGAACCT	61.5
Sucrose phosphate Synthase 3	SPS3	AGCCACATTGAGTCCCCAACGG	65.07	TGCGAGGCCTACGTTGTCATCCT	65.98
Transcript ID: TRINITY_DN21034_c0_g1_i1	SPS3	AGCCACATTGAGTCCCCAACGG	65.07	TGCGAGGCCTACGTTGTCATCCT	65.98
Ehtylene Respoonsive Trnscription Factor ERF012	ERTF	TCAGCAGAAACTCCACAAGCGA	62.43	TGGTATGACTTGGAGGGGTTGCA	63.77
Transcript ID: TRINITY_DN9541_c0_g1_i1	ERTF	TCAGCAGAAACTCCACAAGCGA	62.43	TGGTATGACTTGGAGGGGTTGCA	63.77

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