Table 5 Gene and primes sequence details of qPCR
Gene Name | Gene ID | Forward primer | Tm | Reverse primer | Tm |
|---|---|---|---|---|---|
Receptor like kinase isoform X1 | RLKI-X1 | ACCAAACTCGGAACTTGATGGAGC | 63.37 | GTAGGCCTAAGTGTTGGAGAAGCA | 62.37 |
Transcript ID: TRINITY_DN54263_c0_g1_i14 | |||||
La-related protein | LRP | GGCCCATTCCATTTCCAGCACG | 64.61 | TGCACATGAGGAAGAAGATGGGGA | 63.71 |
Transcript ID: TRINITY_DN56761_c0_g1_i4 | |||||
Probable peroxygenase 4 (Glycine max)73,458,878 (putative peroxygenase 4 | PPER4 | GCTTCCATCATAAACACTTCGG | 62.13 | AGGAAGGATTGGTGGCTTGGTT | 64.61 |
Transcript ID: TRINITY_DN61766_c0_g1_i1 | |||||
Zinc finger CCH domain containing protein 69 –like isoform X1 | ZF | GAGCCTGTCTGAAAGGGGAGCA | 64.24 | TGCAGCGACTACCATAAGCACA | 62.3 |
Transcript ID: TRINITY_DN56363_c0_g1_i13 | |||||
AP2 like ethylene responsive transcription factor ANT like | AP2TF | ACTGTGGGGTGTGGAGAGTTGCA | 66.09 | GCCCTCTCTTCTTTGCATCCACAGC | 66.04 |
Transcript ID: | |||||
TRINITY_DN55029_c2_g4_i10 | |||||
Glyceraldehyde 3 phosphate dehyadrogenase 2,cytolistic | G3PDC | GCCCTCTGACTCCTCCTTGATAGCA | 65.42 | GGCATTCCGTGTCCCTACTGTGGA | 66.28 |
Transcript ID: TRINITY_DN8658_c0_g1_i1 | |||||
Cellulose synthase A catalytic subunit | CS | AACTCACCAGACATCGGTTGCCC | 65.11 | AAGTCGGGGATGCTGTGGGAAGA | 65.72 |
Transcript ID: TRINITY_DN51118_c0_g1_i8 | |||||
Auxin response Factor 3 | ARF3 | CCCATTTCATGTGACTCTTCTG | 63.63 | TGGATTCCAAAGAGCTGAACCT | 61.5 |
Transcript ID: TRINITY_DN22465_c0_g1_i1 | |||||
Sucrose phosphate Synthase 3 | SPS3 | AGCCACATTGAGTCCCCAACGG | 65.07 | TGCGAGGCCTACGTTGTCATCCT | 65.98 |
Transcript ID: TRINITY_DN21034_c0_g1_i1 | |||||
Ehtylene Respoonsive Trnscription Factor ERF012 | ERTF | TCAGCAGAAACTCCACAAGCGA | 62.43 | TGGTATGACTTGGAGGGGTTGCA | 63.77 |
Transcript ID: TRINITY_DN9541_c0_g1_i1 |