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Table 2 Callus induction and shoot regeneration rates under the different cytokinin and auxin combinations listed in Table 1

From: Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants

Condition

Explants with callus formation (%)

Callus condition

Explants with regeneration (%)

Shoot Condition

A

87.4 ± 2.5

Friable

5.0 ± 1.4

+

B

99.2 ± 0.8

Friable

22.8 ± 2.6

+ +

C

89.1 ± 5.1

Compact

29.4 ± 2.9

+ + + + +

D

98.3 ± 0.8

Compact

65.8 ± 3.6

+ + + +

E

95.0 ± 3.8

Compact

53.3 ± 5.1

+ + + + +

F

96.7 ± 3.3

Compact

53.3 ± 5.8

+ + + + +

F-light

95.8 ± 1.7

Compact

29.5 ± 7.7

+ + + +

  1. Values are mean ± SE of technical triplicates with n = 40
  2. The shoot condition was scored based on their appearance (+: Most shoots appear watery, browning or deformed, + + + + +: Most shoots appear strong and healthy)