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Table 2 Substrate specificity of Gossypium hirsutum amidohydrolases

From: The effects of mepiquat chloride on the lateral root initiation of cotton seedlings are associated with auxin and auxin-conjugate homeostasis

Conjugate

Hydrolase activitya (μmol IAA released/min/mg)

His-GhILR1

GST-GhIAR3a

GST-GhIAR3b

IAA-Ala

-b

79±4.5

-

IAA-Asp

4.9±0.5

0.2±0.1

0.5±0.1

IAA-Gly

5.1±0.2

4±0.4

4.6±0.5

IAA-Ile

-

-

-

IAA-Leu

10±1.2

4.3±0.7

4.2±0.5

IAA-Met

6.3±1.0

8.2±0.9

3.8±0.3

IAA-Phe

12.3±1.6

5.1±0.8

5.4±0.7

  1. aValues shown are the means of at least 3 time points ±S.D.s
  2. bNot detected